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BACTERIORHODOPSIN AND THE SENSORY PIGMENT OF THE PHOTOSYSTEM 565 IN HALOBACTERIUM HALOBIUM
Author(s) -
Schimz Angelika,
Sperling Walter,
Hildebrand Eilo,
Köhlerhahn Dorothea
Publication year - 1982
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1982.tb04363.x
Subject(s) - bacteriorhodopsin , action spectrum , pigment , retinal , photochemistry , halobacterium , photosystem ii , chemistry , photosystem i , absorption spectroscopy , absorption (acoustics) , photosynthesis , biophysics , photosystem , rhodopsin , halobacteriaceae , halobacterium salinarum , biochemistry , biology , membrane , materials science , optics , organic chemistry , physics , composite material
Blocking in vivo synthesis of retinal by addition of nicotine to the culture medium leads to the loss of photobehavior in Halobacterium halobium. Addition of rrans ‐retinol or frans‐retinol 2 (3,4‐dehy‐droretinol) restores the responses to light decreases in the green‐yellow spectral range. Action spectra of the reconstituted retinal‐ and retinal 2 ‐photosystem show maximal sensitivity at 565 and 580 nm, respectively. Addition of retinol or retinol 2 also restores the formation of bacteriorhodopsin (BR) or bacteriorhodopsin 2 (BR 2 = 3,4‐dehydroretinal‐bacterio‐opsin complex). The absorption spectra of BR and BR 2 , measured in isolated membranes, as well as in living bacteria, show maxima at 568 nm (BR) and at about 600 nm (BR 2 ), respectively. Comparison of the action spectrum of the retinal2‐containing sensory photosystem with the absorption spectrum of BR 2 suggests that a retinal pigment different from BR is responsible for the photosensory behavior to green‐yellow light.