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A COMPARISON OF THERMALLY INDUCED CHANGES IN SONICATED PHOSPHOLIPID AND SURFACTANT VESICLES BY MEANS OF INTRA‐MOLECULAR EXCIMER‐FORMING PROBE
Author(s) -
Lukac Sava
Publication year - 1982
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1982.tb04333.x
Subject(s) - vesicle , pulmonary surfactant , phospholipid , bilayer , chemistry , sonication , excimer , bromide , lipid bilayer , chromatography , biophysics , membrane , organic chemistry , fluorescence , biochemistry , biology , physics , quantum mechanics
The molecule (1,l'‐dipyrenyl)‐methyl ether (dipyme) was used for monitoring the bilayer fluidity of surfactant and sonicated phospholipid vesicles. In the latter systems, the observed transition temperatures ( Tc ) are identical with those found by different methods. Surfactant vesicles prepared from dioctadecyldimethylammonium bromide (DODAB) and dihexadecylphosphate (DHP) molecules manifest a similar fluidity of their bilayers as those of sonicated phospholipid vesicles below their Tc. However, unlike in phospholipid vesicles, there was no significant change of the bilayer structure above Tc observed in surfactant vesicles. DHP vesicles formed in pure water provide a different solubilization site for dipyme than those prepared in a buffer solution. Such sites are characterized by a relatively high local concentration of the probe and the appearance of the blue shifted spectrum of the excimer.