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PHOTOREACTIVATING ENZYME INDUCTION IN HUMAN LYMPHOCYTES
Author(s) -
Sutherland Betsy M.,
Castellani Amleto
Publication year - 1982
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1982.tb03847.x
Subject(s) - lymphoblast , enzyme , lactate dehydrogenase , biology , stimulation , microbiology and biotechnology , enzyme inducer , enzyme assay , biochemistry , specific activity , dehydrogenase , cell culture , genetics , endocrinology
— Photoreactivating enzyme (PRE) activity was determined in human lymphocytes cultured in the presence or absence of the mitogen phytohemagglutinin (PHA). Photoreactivating enzyme activity increased from 0 to 12pmol/mg/h in about 40 h in cells cultured in the presence of PHA; enzyme activity was unchanged in the absence of PHA. The kinetics of induction of PRE and of lactate dehydrogenase, a marker of mitogenic stimulation, were indistinguishable, indicating that PRE induction results from stimulation of transcription and translation by the mitogen, rather than a specific effect of the PHA on PRE production. The level of PRE was also higher in mouse lymphoblasts growing rapidly in RPMI 1640 medium than in the same cells growing more slowly in Dulbecco's medium.