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THE EFFECT OF MEMBRANE FATTY ACID COMPOSITION ON THE NEAR‐UV (300–400 nm) SENSITIVITY OF ESCHERICHIA COLI K1060
Author(s) -
Klamen Debra L.,
Tuveson R. W.
Publication year - 1982
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1982.tb03827.x
Subject(s) - escherichia coli , fatty acid , double bond , membrane , auxotrophy , chemistry , cyclopropane , molecule , carbon fibers , biochemistry , organic chemistry , materials science , ring (chemistry) , composite number , composite material , gene
— The near‐UV (NUV, 300 400 nm) sensitivity of logarithmically growing Escherichia coli cells of the fatty acid auxotroph K1060 increases with the number of carbon‐carbon double bonds in the fatty acid used as supplement. Cultures of K.1060 grown to stationary phase on unsaturated fatty acids of the same chain length but differing in the number of carbon‐carbon double bonds per molecule differed only marginally in their NUV sensitivity. The clear NUV‐sensitizing effect of increasing double bonds in the fatty acid supplement used to support logarithmic growth implies that the membrane may be an important NUV target only for logarithmically growing cells. Based on these observations, our previous suggestion that inefficient conversion of fatty acids in the membrane to their cyclopropane analogs as an explanation for the NUV‐sensitizing effect of the nur mutation on stationary E. coli cell populations must be wrong.