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PHYSICAL AND CHEMICAL PROPERTIES OF CHLOROPHYLL RCI EXTRACTED FROM PHOTOSYSTEM I OF SPINACH LEAVES AND FROM GREEN ALGAE
Author(s) -
Dornemann Dieter,
Senger Horst
Publication year - 1982
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1982.tb02653.x
Subject(s) - spinach , chlorophyll , chlorophyll a , photosystem i , light harvesting complexes of green plants , photosystem ii , chloroplast , p700 , chlorophyll fluorescence , chlorophyll c , scenedesmus , green algae , botany , fluorescence , photosynthesis , biology , chemistry , algae , biochemistry , physics , optics , gene
— A new chlorophyll, designated chlorophyll RCI (Chi RCI), with absorption and fluorescence properties different to other known chlorophylls, has been extracted from photosystem I (PSI) sub‐chloroplast particles of the green alga Scenedesmus obliquus; it was suggested that this chlorophyll is either the chromophore ofP–700 or the chromophore of another holochrome associated in a 1:1 molar ratio withP–700. We now report the extraction and isolation of a chlorophyll from PSI particle preparations from spinach leaves with properties identical to those of Chi RCI from Scenedesmus. Its molar ratio toP–700 measured in vivo is again approximately 1:1. Chlorophyll RCI is further characterized by its fluorescence characteristics and redox behaviour. Molecular weight determinations show that Chi RCI has a mol wt 35 units higher than that of chlorophyll a (Chi a).