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DNA SYNTHESIS ON ØX174 TEMPLATE DAMAGED BY PROFLAVINE and LIGHT TREATMENT
Author(s) -
Piette J.,
Moore P. D.
Publication year - 1982
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1982.tb02633.x
Subject(s) - proflavine , dna , guanine , chemistry , nucleotide , escherichia coli , microbiology and biotechnology , residue (chemistry) , biochemistry , biology , gene
— Onprimed–0X174 single‐stranded DNA photosensitized in the presence of proflavine, Escherichia coli DNA polymerase terminates DNA synthesis at the nucleotide preceding a guanine residue. This chain termination is visualized on a DNA sequencing gel. Our results show that (i) the major targets of the action of proflavine in the DNA are the guanine residues, (ii) there seems no preferential alteration of some residues and (iii) the rate of alteration is very high. We show that the loss of infectivity of 0X174 mediated by the photodynamic action of proflavine can be explained by a block in the DNA polymerisation reaction.