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BOVINE OCULAR SQUAMOUS CELL CARCINOMA: UV SENSITIVITY IN LYMPHOCYTES
Author(s) -
Lavin Martin F.,
Jennings Philip A.,
Hughes Darryl J.
Publication year - 1982
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1982.tb02630.x
Subject(s) - trypan blue , phytohaemagglutinin , microbiology and biotechnology , dna synthesis , cell culture , biology , cell , radiosensitivity , radiation sensitivity , basal cell , dna , lymphocyte , cancer research , immunology , genetics , pathology , irradiation , medicine , physics , nuclear physics , radiation therapy
— Increased sensitivity to UV light has been demonstrated in Phytohaemagglutinin stimulated lymphocytes from normal and tumour‐bearing Hereford cattle when compared to lymphocytes from other breeds. Trypan blue exclusion and inhibition of DNA synthesis were used to determine cell viability. The results obtained from time course and radiation dose experiments demonstrate biphasic survival kinetics. This is indicative of at least two separate cell populations, exhibiting differentia! sensitivity to UV. The increased sensitivity to UV observed in Herefords may reflect a general sensitivity to UV or alternatively a different cellular constitution in the mitogen stimulated cultures. DNA repair synthesis, measured in the presence of hydroxyurea, was of similar levels in cell cultures from Herefords and one of the control breeds.