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ENHANCEMENT OF ULTRAVIOLET OR N‐METHYL‐N'‐ NITRO‐N‐NITROSOGUANIDINE SENSITIVITY OF DICTYOSTELIUM DISCOIDEUM BY 3‐AMINOBENZAMIDE
Author(s) -
Ohnishi T.,
Eimoto H.,
Okaichi K.
Publication year - 1982
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1982.tb02602.x
Subject(s) - pyrimidine dimer , dictyostelium discoideum , dna repair , dna , methylnitronitrosoguanidine , nucleotide excision repair , mutation , microbiology and biotechnology , biology , ultraviolet light , ultraviolet irradiation , mutagenesis , chemistry , irradiation , biochemistry , photochemistry , gene , physics , nuclear physics
— The amoeboid cells of Dktyostelium discoideum NC–4 possess a 3‐aminobenzamide(3‐ABA)‐sensitive repair mechanism for DNA damages induced by UV‐irradiation or N‐methyl‐N'‐nitro‐N‐nitrosoguanidine (MNNG)‐treatment. We have studied the effect of 3‐ABA on each step of excision repair in the UV‐ irradiated cells. Although the nicking of DNA‐strand and the excision of pyrimidine dimcrs are insensitive to 3‐ABA, the rejoining of DNA strand‐breaks is sensitive. The frequency of mutation induced by UV‐irradiation or MNNG‐treatment is depressed by 3‐ABA. The mechanisms of repair inhibition by 3‐ABA are discussed.