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ULTRAVIOLET SENSITIVITY OF THE ADDITION, DELETION and REPLACEMENT OF LONG NONHOMOLOGOUS DNA SEGMENTS BY GENETIC TRANSFORMATION OF HAEMOPHILUS INFLUENZAE
Author(s) -
Walter Ronald B.,
Stuy Johan H.
Publication year - 1982
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1982.tb02571.x
Subject(s) - prophage , insert (composites) , transformation (genetics) , haemophilus influenzae , plasmid , dna , genetics , biology , mutation , point mutation , materials science , gene , escherichia coli , bacteria , bacteriophage , composite material
— We have recently described the construction and some properties of Haemophilus influenzae Rd strains with long and different R plasmid‐derived DNA segments (nonhomologous inserts) at the same site in the HP1 prophage. These inserts can be added to a recipient's genome by genetic transformation, they can be deleted from the recipient genome, or they can be replaced by another insert. We report here that the UV inactivation of all three phenomena followed single hit kinetics. Deletion was roughly 10 times more resistant; its UV‐sensitivity equaled that of a high‐efficiency point mutation. There was an inverse correlation between UV‐sensitivity and additive transformation efficiency of the various inserts; sensitivity may thus be a measure of insert size. This correlation was not seen for deletion. All three phenomena were more sensitive when they were measured on excision repair‐deficient uvr ‐ recipients. The dose‐reduction factor for addition was about 1.5 while it was about 2.6 for deletion.