Premium
ULTRAVIOLET RADIATION INDUCED CHANGES IN MACROPHAGE ACTIVITY
Author(s) -
Schuller Georgia B.,
Hellman Kiki B.
Publication year - 1981
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1981.tb09072.x
Subject(s) - phagocytosis , macrophage , petri dish , ultraviolet radiation , microbiology and biotechnology , chemistry , viability assay , biology , in vitro , immunology , andrology , biochemistry , medicine , radiochemistry
— The effect of ultraviolet (UV) radiation on macrophage activity was examined. Thioglycollate‐stimulated peritoneal exudate cells were collected from adult C57BL/6 mice. Ninety‐five per cent of the cells adhering to plastic petri dishes were macrophages as determined by the presence of a non‐specific esterase. Adherent cells were exposed to UV radiation of 0.5‐13.2 J/m 2 . Viability and phagocytosis were measured at 0, 24, 48, 72 and 96 h after exposure. A statistically significant UV exposure‐dependent decrease in macrophage viability and phagocytic capacity was observed. Macrophage viability and phagocytosis also decreased as a function of time after exposure to UV radiation.