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LINEAR AND CIRCULAR DICHROISM AND FLUORESCENCE POLARIZATION OF THE B875 LIGHT‐HARVESTING BACTERIOCHLOROPHYLL‐PROTEIN COMPLEX FROM RHODOPSEUDOMONAS SPHAEROIDES
Author(s) -
Bolt John D.,
Hunter C. Neil,
Niederman Robert A.,
Sauer Kenneth
Publication year - 1981
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1981.tb09057.x
Subject(s) - bacteriochlorophyll , linear dichroism , rhodobacter sphaeroides , fluorescence , circular dichroism , chemistry , fluorescence anisotropy , rhodopseudomonas , light harvesting complex , circular polarization , biophysics , photochemistry , crystallography , optics , biology , physics , photosynthesis , biochemistry , photosystem ii , microstrip
— The orientation of the chromophores in the B875 light‐harvesting bacteriochlorophyll complex isolated from Rhodopseudomonas sphaeroides by lithium dodecyl sulfate/polyacrylamide gel electrophoresis was examined by linear and circular dichroism and fluorescence polarization procedures. The circular dichroism in the near‐IR was weaker than that of the B800–850 light‐harvesting complex and had a distinctly different shape. This suggested a different geometry for the two bacteriochlorophylls of B875 and less interactive association between their transition moments. The magnitude of the circular dichroism in the carotenoid region of B875 was similar to that of B800–850 but gave more negative values between approx. 430–485 nm; this may reflect a difference in the asymmetric binding of carotenoids to the B875 protein. The fluorescence polarization increased sharply across the near‐IR region of B875 and achieved very high values at long wavelengths. This confirmed that more than one transition contributed to this absorption band. The linear dichroism of B875 did not show a significant change in this near‐IR band like that observed for the longest wavelength band of B800–850. Thus, the transition moments for each bacteriochlorophyll within B875 appear to be tilted to approximately the same extent with respect to the protein axis. These results distinguish B875 from all other light‐harvesting complexes and suggest that the antennae of Rhodospirillaceae which contain a single near‐IR absorption band cannot be classified into a single group.