Premium
PHYCOBILISOMES AND ISOLATED PHYCOBILIPROTEINS. EFFECT OF GLUTARDIALDEHYDE AND BENZOQUINONE ON FLUORESCENCE
Author(s) -
Köst H.P.,
Rath K.,
Wanner G.,
Scheer H.
Publication year - 1981
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1981.tb08976.x
Subject(s) - phycobiliprotein , phycobilisome , phycoerythrin , fluorescence , phycocyanin , allophycocyanin , chemistry , kinetics , photochemistry , cyanobacteria , biology , microbiology and biotechnology , bacteria , flow cytometry , optics , genetics , physics , quantum mechanics
— The fluorescence of the biliproteins C‐phycocyanin from Spirulina platensis , B‐phycoerythrin from Porphyridium cruentum and of isolated whole P. cruentum phycobilisomes is quenched in the presence of glutardialdehyde (GA) or benzoquinone (BQ). The kinetics of fluorescence decrease thus induced is biphasic. If GA is used as a quencher, the fluorescence can be recovered at 77 K. Contrary to the GA‐effect, only a minor recovery takes place with BQ at 77 K, thus demonstrating a different mechanism of action of GA and BQ on biliproteins.