Premium
NEAR‐UV INDUCTION OF SISTER CHROMATID EXCHANGES UNDER AEROBIC AND ANAEROBIC CONDITIONS
Author(s) -
Griego Viola M.,
Matsushita Geraldine C.,
Matsushita Tatsuo
Publication year - 1981
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1981.tb08960.x
Subject(s) - sister chromatids , anaerobic exercise , chemistry , sister , biology , biochemistry , physiology , gene , chromosome , sociology , anthropology
— The induction of sister chromatid exchanges (SCE) and cell sensitivity in mouse myeloma cells (66.2 subclone of MPC11) by irradiation with monochromatic near‐UV (365 nm) light were studied under aerobic and anaerobic conditions. Sister chromatid exchanges were studied using the fluorescence‐plus‐Giemsa technique, and sensitivity was determined by the ability of irradiated and nonirradiated control cells to form colonies in soft agar. Cells were found to be 16 times more sensitive to near‐UV light under aerobic exposure, producing an F 37 value of 7 × 10 4 J/m 2 compared to the F 37 value of 11.5 × 10 5 J/m 2 under anaerobic conditions. The induction of SCE was also 12 times more efficient for aerobic irradiation than for anaerobic irradiation. The data suggest that the SCE‐inducing potential of DNA lesions differs when near‐UV irradiation is performed in the presence or absence of air. In addition, the DNA lesions responsible for lethality and also those lesions leading to SCE induction may differ under the two irradiation conditions.