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UV‐ENHANCED REACTIVATION OF MINUTE‐VIRUS‐OF‐MICE: STIMULATION OF A LATE STEP IN THE VIRAL LIFE CYCLE
Author(s) -
Rommelaere Jean,
Vos JeanMichel,
Crnelis Jan J.,
Ward David C.
Publication year - 1981
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1981.tb05502.x
Subject(s) - virus , multiplicity of infection , biology , viral replication , parvovirus , minute virus of mice , irradiation , virology , stimulation , viral life cycle , microbiology and biotechnology , parvoviridae , physics , neuroscience , nuclear physics
— UV‐enhanced reactivation of minute‐virus‐of‐mice (MVM), an autonomous parvovirus, was studied in parasynchronous mouse A9 cells. The survival of UV‐irradiated MVM is increased in cells which have been UV‐irradiated prior to infection. UV‐enhanced reactivation can be explained neither by facilitated plaque detection on UV‐treated indicator cells, nor by altered kinetics of virus production by UV‐irradiated cells. No effect of the multiplicity of infection on virus survival was detected in unirra‐diated or irradiated cells. The magnitude of UV‐enhanced reactivation is a direct exponential function of the UV dose administered to the virus while virus survival is inversely proportional to the UV dosage. The expression of UV‐enhanced reactivation can be activated in cells arrested in G 0 , it requires de novo protein synthesis and it is maximal when cells are irradiated 30 h before the onset of viral DNA replication. Early phases of the viral cycle, such as adsorption to cellular receptors, migration to the nucleus and uncoating, were not affected by cell irradiation and are unlikely targets of the UV‐enhanced reactivation function(s). These results, together with the single‐strandedness of the viral genome, strongly suggest that the step stimulated in UV‐irradiated cells functions concomitant with, or subsequent to, viral DNA replication.