Premium
SELECTIVE INACTIVATION OF MICROSOMAL DRUG METABOLIZING PROTEINS BY VISIBLE LIGHT
Author(s) -
Augusto Ohara,
Packer Lester
Publication year - 1981
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1981.tb05488.x
Subject(s) - cytochrome , chemistry , microsome , reductase , biochemistry , lipid peroxidation , hemeprotein , cytochrome c , microsoma , enzyme , heme , mitochondrion
— Rat liver microsomes treated with heterochromatic visible light (λ > 400 nm) and O 2 showed a preferential inactivation of NADPH‐cytochrome P 450 reductase and cytochrome P 450 , proteins involved in drug metabolism. Cytochrome P 450 destruction correlates with lipid peroxidation; both are oxygen dependent and affected to the same extent by antioxidants and radical scavengers. Under anaerobic conditions. NADPH‐cytochrome P 450 reductase is the only component which is inactivated; this is accompanied by FMN loss and activity can be restored by reconstitution.