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INHIBITION OF RESPIRATION AND LOSS OF MEMBRANE INTEGRITY BY SINGLET OXYGEN GENERATED BY A PHOTOSENSITIZED REACTION IN NEUROSPORA CRASSA CONIDIA
Author(s) -
ShimizuTakahama Michiko,
Egashira Takeshi,
Takahama Umeo
Publication year - 1981
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1981.tb05475.x
Subject(s) - neurospora crassa , conidium , singlet oxygen , neurospora , respiration , photochemistry , oxygen , chemistry , biophysics , biochemistry , biology , botany , organic chemistry , mutant , gene
— It was found that killing of Neurospora crassa conidia by singlet molecular oxygen generated by a photosensitized reaction in the presence of toluidine blue O, accompanied inhibition of respiration and uptake of the photosensitizing dye by conidia. Both the inactivation of respiration and the uptake of toluidine blue O were stimulated by deuterium oxide and suppressed by azide. Mitochondria of irradiated conidia were swollen and showed matrix of low density under an electron microscope. These results suggest that singlet molecular oxygen induces the inhibition of respiration and the loss of membrane integrity of Neurospora conidia.