Premium
PHOTOREACTION OF THE ACIDIFIED FORM OF BACTERIORHODOPSIN AND ITS 9‐ CIS DERIVATIVE IN PURPLE MEMBRANE AT LOW TEMPERATURES
Author(s) -
Maeda Akio,
Iwasa Tatsuo,
Yoshizawa Tǒru
Publication year - 1981
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1981.tb05459.x
Subject(s) - bacteriorhodopsin , chemistry , chromophore , halobacteriaceae , isomerization , halobacterium , photochemistry , photoisomerization , derivative (finance) , membrane , stereochemistry , organic chemistry , biochemistry , halobacterium salinarum , financial economics , economics , catalysis
— The photoreaction of the acidified form of bacteriorhodopsin and its 9‐cis derivative was studied by low temperature spectroscopy. A short exposure of the acidified form of bacteriorhodopsin, which was prepared by adding 2 m M HC1 to purple membrane suspension in 67% glycerol at 0°C, to red light at – 72°C resulted in the blue‐shift of the spectrum. The feature of the shift was very similar to that accompanied by the formation of stable 9‐ cis acidified form of bacteriorhodopsin at 0°C, but only 13‐ cis ‐ and all‐ trans ‐retinals were found in the extract from this product. No blue‐shifted product was found on irradiation at – 190°C. Irradiation of the 9‐ cis form of acidified bacteriorhodopsin at ‐72°C with blue light caused the isomerization of its 9‐ cis ‐retinylidene chromophore to 13‐ cis and all‐ trans forms without a significant spectral change. It became greater only after the sample was warmed above – 24°C. These results indicate the presence of the light‐induced product which has trans configuration on the 9‐10 double bond and exhibits the 9‐ cis type spectrum.