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INACTIVATION OF BACTERIOPHAGE LAMBDA BY NEAR–ULTRAVIOLET IRRADIATION IN THE PRESENCE OF CHLORPROMAZINE
Author(s) -
Fujita Hitoshi,
Endo Akira,
Suzuki Kenshi
Publication year - 1981
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1981.tb05327.x
Subject(s) - chemistry , bacteriophage , irradiation , centrifugation , dna , breakage , anaerobic exercise , radiochemistry , photochemistry , biophysics , biochemistry , biology , escherichia coli , physiology , physics , materials science , nuclear physics , composite material , gene
Bacteriophage λ vir was inactivated when it was irradiated with near‐UV light in the presence of chlorpromazine. DNA strand breakage in the treated phage was indicated by alkaline sucrose gradient centrifugation. The number of the breaks was increased with increasing fluence. Although the inactivation rate was enhanced with a decreasing salt concentration in the reaction mixture and under a nitrogen atmosphere, the number of the strand breaks was not altered in either case. Therefore, the DNA strand breakage is not a sole lethal damage in the treated phage. The addition of NaN 3 repressed the inactivation and the reaction in a D 2 O medium enhanced the inactivation even if the reaction mixture was irradiated under anaerobic conditions. Under anaerobic conditions, the inactivation occurs presumably via a radical mechanism.