Premium
THE SENSITIVITY OF TRICHOMONAS VAGINALIS AND TRICHOMONAS GALLINAE TO ULTRAVIOLET RADIATION
Author(s) -
Daly James J.,
Baker Max L.,
Burton Stanley B.
Publication year - 1981
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1981.tb05323.x
Subject(s) - trichomonas vaginalis , hemocytometer , stationary phase , biology , trichomonas , ultraviolet , irradiation , exponential growth , microbiology and biotechnology , chemistry , chromatography , optics , biochemistry , physics , nuclear physics , quantum mechanics
The formation of colonies in solid medium was used as a criterion of viability to determine the effect of ultraviolet radiation on Trichomonas vaginalis. Both viability (colony) counts and total cell (hemocytometer) counts were used to estimate physiological ages of cell populations to be irradiated. Washed‐cell suspensions in 0.6% saline were exposed to far‐ (254 nm) and near‐UV (300–400 nm) radiation and dose‐response survival curves were constructed from colony counts. The effect of far‐UV was found to be independent of growth phase with the D 0 for exponential, early stationary, and late stationary cells 2.6, 2.7, and 2.7 J/m 2 , respectively. Survival to near‐UV increased with the age of cells with the estimated D 50 being 216 J/m 2 for exponential cells, 1360 J/m 2 for early stationary cells, and 4200 J/m 2 for late stationary cells. Exponential cells of Trichomonas gallinae irradiated with near‐UV had a D 50 of 340 J/m 2 . T. vaginalis is highly sensitive to far‐UV relative to protozoa. T. vaginalis and T. gallinae are highly sensitive to near‐UV relative to other microorganisms.