Premium
DOES MEMBRANE ATTACKING PHOTODYNAMIC ACTION REFLECT THE SO‐CALLED PHASE TRANSITION?
Author(s) -
Ito Takashi
Publication year - 1981
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1981.tb04307.x
Subject(s) - toluidine , saccharomyces cerevisiae , membrane , incubation , photosensitizer , solvent , chemistry , phase transition , biophysics , cell membrane , photochemistry , stationary phase , biochemistry , yeast , biology , physics , chromatography , organic chemistry , condensed matter physics
Abstract— With Saccharomyces cerevisiae cells the temperature dependence of toluidine blue (TB) sensitized photodynamic inactivation was measured in the range 10–33°C using either H 2 O (pH 7.9) or 45% D 2 O (pH 6.8) as a solvent. Plots of ln(1/LD 50 ) (LD 50 : light dose for 50% survival) as a function of the reciprocal of temperature could be fitted by two straight lines with a discontinuity at 21–22°C. In view of previous findings on TB‐cell interaction that TB sensitizes the cell, after long incubation, in the membrane rather than from outside the cell, it is suggested that the present results reflect the phase transition of lipid matrix.