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ACTION SPECTRA OF CATION EFFECTS ON THE FLUORESCENCE POLARIZATION AND INTENSITY IN THYLAKOIDS AT ROOM TEMPERATURE
Author(s) -
Wong Daniel,
Ovindjee G
Publication year - 1981
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1981.tb04304.x
Subject(s) - fluorescence , excitation , chemistry , degree of polarization , photosystem ii , polarization (electrochemistry) , thylakoid , photochemistry , fluorescence anisotropy , chlorophyll a , photosynthetic reaction centre , wavelength , analytical chemistry (journal) , spectral line , action spectrum , depolarization , photosynthesis , biophysics , optics , materials science , chloroplast , optoelectronics , physics , electron transfer , biology , scattering , biochemistry , astronomy , quantum mechanics , chromatography , gene
— The degree of polarization of chlorophyll‐ a (Chl‐ a ) fluorescence is known to monitor the extent of excitation migration and/or the orientation of the photosynthetic pigment molecules. We report here the effects of cations, at room temperature, on the degree of polarization of Chl‐ a fluorescence, and fluorescence intensity in thylakoids as a function of excitation wavelength. Observations of maxima at 650 and 675 nm in the cation‐induced changes in the excitation spectrum for fluorescence at 730 and 762 nm, and, in the action spectra for the depolarization of fluorescence lead us to suggest that the regulation of the initial distribution of excitation to photosystem II involves the better coupling of Chl‐ b and‐ a in the light harvesting complex with Chl‐ a in the reaction center II complex.