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OBSERVATION OF THE PICOSECOND TIME‐RESOLVED SPECTRUM OF SQUID BATHORHODOPSIN AT ROOM TEMPERATURE
Author(s) -
Suzuki Kaori,
Kobayashi Takayoshi,
Ohtani Hiroyuki,
Yesaka Hiroshi,
Nagakura Saburo,
Shichida Yoshinori,
Yoshizawa Tôru
Publication year - 1980
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1980.tb04060.x
Subject(s) - squid , picosecond , rhodopsin , spectrometer , analytical chemistry (journal) , chemistry , excitation , spectral line , materials science , optics , laser , physics , retinal , ecology , biochemistry , chromatography , astronomy , biology , quantum mechanics
— Difference spectra between squid rhodopsin and its bathorhodopsin at room temperature were measured ca. 150 ps and ca. 500 ps after the excitation at 347.2 nm by a double‐beam picosecond time‐resolved spectrometer. The spectra measured showed a red shift of the isosbestic point between squid rhodopsin and its bathorhodopsin and a lower Δ A max /Δ A min value compared with those measured at low temperatures by conventional spectrophotometry.