Premium
EXISTENCE OF HYPSORHODOPSIN AS THE FIRST INTERMEDIATE IN THE PRIMARY PHOTOCHEMICAL PROCESS OF CATTLE RHODOPSIN
Author(s) -
Kobayashi Takayoshi
Publication year - 1980
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1980.tb04011.x
Subject(s) - rhodopsin , picosecond , intermediate state , photochemistry , chemistry , reaction intermediate , reaction rate constant , excited state , reactive intermediate , process (computing) , kinetics , optics , laser , organic chemistry , catalysis , retinal , atomic physics , biochemistry , physics , quantum mechanics , computer science , operating system
— It has long been believed that bathorhodopsin is the first intermediate of visual process for cattle rhodopsin. In the present paper hypsorhodopsin is shown to be the first intermediate by the use of picosecond spectropic technique. The main first intermediate, hypsorhodopsin, is formed with the time constant of 15 ± 5 ps. The time constant of the formation of bathorhodopsin from hypsorhodopsin is 50 ± 20 ps. Bathorhodopsin intermediates formed directly from excited state rhodopsin and those formed indirectly through hypsorhodopsin are 71/2 # % and 93%, respectively, of total bathorhodopsin intermediates in octylglucoside buffered solution. Batho intermediates formed directly and indirectly are 0% and 100%. respectively, of total batho intermediates in LDAO buffered solution.