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LOSS OF PHOTOREACTIVABILITY DURING LIQUID‐HOLDING IN ESCHERICHIA COL1 WP2
Author(s) -
Roczen Diktmar,
Mennigmann HorstDieter
Publication year - 1980
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1980.tb04008.x
Subject(s) - photolyase , escherichia coli , strain (injury) , chemistry , biology , biochemistry , dna repair , dna , anatomy , gene
— Loss of photoreactivability during liquid‐holding occurs much faster in Escherichia coli WP2 than has been reported for another strain of B/r (Harm, 1968). The underlying mechanism considerably reduces the maximum photoreactivation attainable within 30 min. The addition of caffeine to UV irradiated strain WP2 prevents the loss of photoreactivation activity. Similarly, a uvr A mutant also fails to show this loss of photoreactivation capacity. These results are consistent with the view that in WP2 the initial steps of excision repair take place during liquid‐holding and that they can preclude photoreactivation at a high rate. But it remains to be elucidated why different strains of B/r differ with respect to this rate.