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EFFECT OF INTRACELLULAR INJECTION OF EGTA AND TETRAETHYLAMMONIUM CHLORIDE ON THE RECEPTOR POTENTIAL OF LOCUST PHOTORECEPTORS
Author(s) -
Tsukahara Yasuo
Publication year - 1980
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1980.tb03796.x
Subject(s) - egta , tetraethylammonium , locust , biophysics , intracellular , depolarization , chemistry , tetraethylammonium chloride , membrane potential , resting potential , reversal potential , phase (matter) , receptor potential , conductance , calcium , potassium , botany , receptor , biology , biochemistry , patch clamp , mathematics , organic chemistry , combinatorics
— The depolarizing photoreceptor potential of locust, Valanga irregularis , as that of other arthropods, has an initial transient phase that declines to a stable sustained phase. In this work, the effect of intracellular injection of EGTA and TEA on these phases was studied. (1) Reversal potential of the transient phase was higher than that of the sustained phase. (2) EGTA and TEA enhanced both phases and the enhancement was more prominent in the sustained phase. (3) Resting potential was shifted slightly upward by the injection. The amplitude of the maximum response was also increased. (4) Current injected through an electrode filled with 2 M KCl had a significant effect on the shape of the response depending on the direction of the current. (5) It is suggested that the activation of K + conductance induced by an increase of intracellular free Ca 2+ concentration causes a decrease in amplitude of the sustained phase.