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GRAPHICAL ESTIMATION OF CROSS SECTIONS FROM FLUENCE‐RESPONSE DATA
Author(s) -
Lipson Edward D.,
Presti David
Publication year - 1980
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1980.tb03778.x
Subject(s) - absorbance , flavin group , biological system , phycomyces , chemistry , fluence , neurospora , photochemistry , biophysics , chromatography , biochemistry , biology , organic chemistry , ion , gene , mutant , neurospora crassa , enzyme
— Light‐induced absorbance changes have been studied by several investigators as a potential assay for the blue‐light receptor pigments in numerous organisms. These studies involve a flavin‐mediated photoreduction of a cytochrome. Here we consider several alternative models for the photochemical kinetics linking the flavin excitation to the cytochrome absorbance change. Straightforward approximations from these models form the basis of a simple graphical procedure which, when applied to fluence‐response data, leads to an estimate of the quantum efficiency for the production of the absorbance change. Such estimates are helpful in evaluating the relevance of this spectrophotometric effect to the physiological responses. Efficiencies of order unity are indicated for results on Neurospora and corn compared to a much lower efficiency for a mycelial preparation of Phycomyces.