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PHOTOREACTIVATION OF ICR 2A FROG CELLS AFTER EXPOSURE TO MONOCHROMATIC ULTRAVIOLET RADIATION IN THE 252–313 nm RANGE
Author(s) -
Rosenstein Barry S.,
Setlow R. B.
Publication year - 1980
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1980.tb03775.x
Subject(s) - photolyase , pyrimidine dimer , endonuclease , action spectrum , monochromatic color , ultraviolet , dna , radiation , ultraviolet radiation , biology , biophysics , dna damage , microbiology and biotechnology , chemistry , dna repair , optics , biochemistry , physics , radiochemistry , botany
— Exposure of ICR 2A frog cells to photoreactivating light after treatment with monochromatic ultraviolet (UV) radiation in the 252–313 nm range resulted in an increase in survival with similar photoreactivable sectors for each of the wavelengths tested. As photoreactivating enzyme is specific for the repair of pyrimidine dimers in DNA, these findings support the hypothesis that these are critical lesions responsible for killing of cells exposed to UV radiation in this wavelength range. The action spectra for cell killing and production of UV‐endonuclease sensitive sites were similar to the DNA absorption spectrum though not identical. Because the number of endonuclease sensitive sites is a reflection of the yield of pyrimidine dimers, these data also suggest that the induction of dimers in DNA by UV radiation in the 252–313 nm range is the principal event leading to cell death.