Premium
TRANSFORMATION OF BALB 3T3 CELLS EXPOSED TO A GERMICIDAL UV LAMP AND A SUNLAMP
Author(s) -
Withrow Thomas J.,
Lugo Maria H.,
Dempsey Mary J.
Publication year - 1980
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1980.tb03696.x
Subject(s) - transformation (genetics) , 3t3 cells , biology , irradiation , chemistry , microbiology and biotechnology , cell culture , genetics , physics , transfection , gene , nuclear physics
— The effect of germicidal UV and sunlamp exposure on direct and simian virus‐40 (SV‐40) transformation of Balb 3T3 cells was studied. Transformation was determined by the ability of transformed cells to grow as clones in agar. Radiation from these lamps enhanced direct transformation, and enhanced viral transformation to approximately the same degree. Enhanced transformation was seen with exposures of light that caused no measurable cell killing, which suggests that the induction of new transformants is involved rather than the selection of pre‐existing transformants. Induction is also suggested by post‐irradiation growth kinetics experiments.