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ACTION SPECTRUM FOR GROWTH DELAY INDUCED BY NEAR‐ULTRAVIOLET LIGHT IN E. coli B/r K*
Author(s) -
Kubitschek H. E.,
Peak M. J.
Publication year - 1980
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1980.tb03682.x
Subject(s) - action spectrum , escherichia coli , chromophore , rna , chemistry , wavelength , pyrimidine , ultraviolet , spectrum (functional analysis) , linkage (software) , photochemistry , physics , stereochemistry , optics , biochemistry , gene , quantum mechanics
Abstract— The action spectrum for growth delay induced by near‐uv light was determined for Escherichia coli B/r growing in a defined medium. This spectrum agrees with and extends that determined earlier by Jagger and his co‐workers for E. coli B growing in nutrient broth. The extended spectrum is indistinguishable from the absorption spectrum for 4‐thiouridine above 320 nm, but deviates significantly at wavelengths shorter than this from the spectrum for 8–13 link formation in transfer RNA containing 4‐thiouridine at position 8. These results extend the evidence that 4‐thiouridine in transfer RNA is the chromophore for near‐UV induction of growth delay, but weaken the case for linkage of a pyrimidine at position 13 in transfer RNA as the mechanism of growth delay.