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TRYPTOPHAN EMISSION FROM HUMAN HEMOGLOBIN AND ITS ISOLATED SUBUNITS
Author(s) -
Alpert Bernard,
Jameson David M.,
Weber Gregorio
Publication year - 1980
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1980.tb03674.x
Subject(s) - hemoglobin , fluorophore , tryptophan , chemistry , hemeprotein , fluorescence , emission spectrum , photochemistry , myoglobin , biophysics , analytical chemistry (journal) , spectral line , biochemistry , heme , chromatography , biology , optics , physics , amino acid , astronomy , enzyme
— The emission spectra of human adult hemoglobin A 0 and its isolated α and ß subunits were obtained using a highly sensitive photon‐counting spectrofluorometer. The quantum yields of the emissions, relative to free tryptophan, were also measured as well as the excitation polarization spectra for hemoglobin A 0 and apohemoglobin. The fluorophore bis‐ANS was utilized to probe for the presence of apoproteins in the hemoprotein preparations. The work suggests that tryptophan may be useful as an intrinsic probe to study dynamical processes in hemoglobin.