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CHARACTERIZATION OF A MICROSECOND INTERMEDIATE IN THE LASER FLASH PHOTOLYSIS OF SMALL PHYTOCHROME FROM OAT
Author(s) -
Braslavsky, Silvia E.,
Matthews, J. Ioan,
Herbert, Hans J.,
Kok Jacob,
Spruit Carel J. P.,
Schaffner Kurt
Publication year - 1980
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1980.tb02562.x
Subject(s) - microsecond , phytochrome , absorption (acoustics) , chromophore , wavelength , flash photolysis , laser , ultrafast laser spectroscopy , irradiation , photochemistry , chemistry , absorption band , transient (computer programming) , absorption spectroscopy , analytical chemistry (journal) , optics , kinetics , physics , botany , reaction rate constant , biology , red light , chromatography , computer science , nuclear physics , operating system , quantum mechanics
— Irradiation of small phytochrome from oat in its P r form with 15 ns laser pulses of different wavelengths(605–655 nm) gave rise to a difference absorption with maxima at 400 and 685 nm for the first detectable transient. Bleaching of a 660 nm band was observed, non‐recuperable up to 1 ms. The transient absorption has a lifetime of 70±15 μs at 273 K. The transient is tentatively identified as lumi‐R and the conformation of its chromophore is postulated to be more extended than that of P r . A deviation from the exponential decay of the lumi‐R absorption at 284 and 300 K and the lack of observable enhancement of the far‐red absorption within 1 ms are interpreted in terms of the appearance of still other intermediates on this time scale between lumi‐R and P fr phytochrome.