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LIGHT AND pH INDUCED CHANGES IN THE SURFACE PRESSURE‐AREA ISOTHERMS OF BACTERIORHODOPSIN
Author(s) -
Vivino A.,
Berns D. S.,
Brody S. S.
Publication year - 1980
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1980.tb02554.x
Subject(s) - bacteriorhodopsin , monolayer , chemistry , surface pressure , aqueous solution , molecule , analytical chemistry (journal) , membrane , chromatography , organic chemistry , biochemistry , mechanics , physics
— Pressure‐area isotherms of monolayers of purple membrane fragments were measured at an air‐water interface. The pH of the aqueous phase was varied from 2 to 10. Over the pH range 5 to 9 the area/molecule of bacteriorhodopsin was constant and illumination of the film had no effect on the isotherms. At pH's more acid than 5 and more alkaline than 9, there were large decreases in area/molecule. Under these conditions of extreme pH the monolayer was very sensitive to light. Illumination resulted in irreversible increases in area/molecule.