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SEASONAL PHOTOSENSITIVITY OF BLEPHARISMA: THE ROLE OF TEMPERATURE
Author(s) -
Glese Arthur C.
Publication year - 1979
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1979.tb09269.x
Subject(s) - sunlight , photosensitivity , daylight , noon , blue light , pigment , fluorescence , red light , fluorescent lamp , photochemistry , near ultraviolet , chemistry , optics , materials science , botany , biology , optoelectronics , physics , atmospheric sciences , organic chemistry
— Red blepharismas ( B. japonicum ) in Pyrex test tubes exposed near noon to clear summer daylight are killed. If similarly exposed at dawn, they turn blue as the red pigment blepharismin is oxidized to oxyblepharismin. and they resist further exposure to daylight. Red blepharismas similarly exposed to sunlight at dawn in winter are killed. The experiments reported seek the reasons for the seasonal difference in photosensitivity and the way in which blepherismas survive winter. Red blepharismas ( B. japonicum ) exposed to visible and near ultraviolet (UV) radiation from two 4W coolwhite fluorescent lamps (5 Wm ‐2 ; 2151ux) resist an 8h exposure between 15° and 34°C. turning blue. Blue and colorless blepharismas also resist 8h exposures to the light at 15° and 34°C. At 8°C both red and blue populations are killed, death appearing to result from deranged water regulation; colorless ones are not affected. Red blepharismas are killed by fluorescent light at 8° C even when the near‐UV radiation is largely excluded by a Corning glass filter; blue and colorless ones are not. The reds are also killed at all temperatures by sunlight filtered through Pyrex glass if exposed near noon on a sunny day. At temperatures above 8° C they are not killed by direct sunlight passed through glass if exposed at dawn, but they turn blue. Blepharismas in fluorescent light divide at the same rate as controls in the dark, but they get smaller. When exposed to sunlight at dawn, at temperatures above 8°C. blepharismas in Pyrex test tubes containing stones or fruiting spikes of grass, hid in the shade of these objects, apparently by random movements. If exposed under similar conditions near noontime, the majority failed to find cover and were killed. Blepharismas survive in summer by avoiding sunlight, in winter by encystment. Colorless protozoans Paraineciurn multimicronucleatum, Colpidium colpoda and Spirostomum teres resisted killing in summer sunlight and with fluorescent light at all temperatures tested.