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TRIPLET‐TRIPLET ENERGY TRANSFER IN P‐TRYPSIN 1 THE ROLE OF THE INDOLE TRIPLET IN THE ULTRAVIOLET‐INDUCED PHOTOLYSIS OF β‐TRYPSIN
Author(s) -
Ramachandran N.,
Ghiron C. A.
Publication year - 1979
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1979.tb09262.x
Subject(s) - chemistry , indole test , trypsin , photochemistry , photodissociation , excited state , energy transfer , side chain , ultraviolet , thiol , stereochemistry , enzyme , biochemistry , organic chemistry , materials science , molecular physics , physics , nuclear physics , polymer , optoelectronics
— Detection of triplet‐triplet energy transfer in an aqueous solution of P‐trypsin is reported. This conclusion is based on the observation that a light excited phenolate side chain can sensitize the destruction of an adjacent indole side chain. The role that the indole triplet might play in the UV‐induced photolysis of /l‐trypsin is also investi‐gated. The results suggest that the UV (309 nm)‐induced inactivation of P‐trypsin is not caused by indole ring destruction but by the disruption of disulfide bonds without thiol formation.