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STRUCTURAL FACTORS CONTROLLING THE ACTION OF UV LIGHT IN NUCLEIC ACIDS *
Author(s) -
Lang H.,
Zimmer Ch.
Publication year - 1979
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1979.tb07824.x
Subject(s) - thymine , cyclobutane , pyrimidine dimer , chemistry , quantum yield , photochemistry , dimer , nucleic acid , irradiation , yield (engineering) , nucleotide , photodissociation , stereochemistry , dna , dna damage , biochemistry , organic chemistry , materials science , fluorescence , ring (chemistry) , physics , quantum mechanics , gene , nuclear physics , metallurgy
— UV light induced conformational effects of different deoxyoligonucleotides and deoxypolynucleotides containing thymine and adenine residues are investigated by means of CD measurements and quantum yield calculations. UV‐irradiation at the wavelengths 254 , 280 and 313 nm indicate that unsensitized irradiation at low doses leads to thymine photoproduct formation of non‐cyclobutane type. In contrast to that irradiation at 313 nm in the presence of acetophenone causes different changes in the CD spectra due to the formation of thymine dimers of the cyclobutane type structure. Quantum yield calculations demonstrate a pronounced dependence of the photoproduct formation on the nucleotide sequence of the oligomers. Thus, clustering of thymine dimer formation can be neglected. Adenine photoproducts in the (A.T) containing oligomers are only formed at higher fluences. > 1.5 × 10 4 J/m 2 and are biological less important events.