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BIOLOGICAL EFFECTS OF NEAR‐UV RADIATION 2‘‐ACETYLFORMANILIDE‐SENSITIZED FORMATION OF HYDROGEN PEROXIDE FROM NUCLEOSIDES AND NUCLEOTIDES
Author(s) -
McCormick J. P.,
Oczos A.
Publication year - 1979
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1979.tb07812.x
Subject(s) - hydrogen peroxide , chemistry , photochemistry , guanosine , singlet oxygen , superoxide , cytidine , nucleotide , uridine , aqueous solution , peroxide , substrate (aquarium) , superoxide dismutase , oxygen , inorganic chemistry , organic chemistry , biochemistry , rna , enzyme , oceanography , gene , geology
Abstraet—2′‐Acetylformanilide has been found to be an effective, near‐ultraviolet (300–380 nm) sensitizer for the photooxidation of nucleosides and nucleotides in aqueous solution, with hydrogen peroxide being formed in high yield. The decreasing order of hydrogen peroxide formation and substrate destruction was found to be: guanosine. adenosine, thymidine, uridine and cytidine. The process was highly pH dependent, low pH being most favorable for photooxidation. Experiments using deuterium oxide and superoxide dismutase indicate that both singlet oxygen and superoxide ion can be involved in hydrogen peroxide formation.