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PHOTOSENSITIZED FORMATION OF RNA‐PROTEIN CROSSLINKS IN AN INSECT EGG ( SMITTZA SPEC., CHIRONOMIDAE, DIPTERA) *
Author(s) -
Jackle Herbert,
Kalthoff Klaus
Publication year - 1979
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1979.tb07811.x
Subject(s) - rna , formamide , dimethyl sulfoxide , chemistry , photosensitizer , irradiation , biochemistry , biology , nuclear chemistry , photochemistry , organic chemistry , gene , physics , nuclear physics
—Upon irradiation of Smittia eggs with ultraviolet light (UV), the extractability of RNA with phenol decreased. The strongest decrease was observed after irradiation at 295 nm wavelength. After trypsin treatment, RNA could be recovered to the same degree as from unirradiated eggs. The extractability of RNA from irradiated eggs was not enhanced by formamide. dimethyl sulfoxide. high salt concentration or heat treatment. The results suggest that the UV‐mediated formation of RNA‐protein crosslinks in Smittia eggs involves the action of a photosensitizer.