Premium
REVISED SPECTRA FOR THE INACTIVATION OF HAEMOPHILUS INFLUENZAE TRANSFORMING DNA BY MONOCHROMATIC ULTRAVIOLET LIGHT: EFFECT OF HISTIDINE
Author(s) -
Peak M. J.,
Tuveson R. W.
Publication year - 1979
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1979.tb07779.x
Subject(s) - histidine , haemophilus influenzae , monochromatic color , ultraviolet , dna , ultraviolet light , chemistry , photochemistry , biophysics , biology , biochemistry , physics , optics , enzyme , antibiotics
—It was reported previously that histidine sensitizes the genetic activity of Haemophilus influenzae transforming DNA to pure 334 nm ultraviolet light, Further measurements show that this apparent 334 nm sensitization was probably erroneous and that in fact, histidine protects DNA against inactivation by 334 nm light. This is now consistent with all previous observations that transforming DNA is protected by histidine against all near‐UV wavelengths (above 320 nm) investigated. A modified spectrum for the protection of H. influenza transforming DNA by histidine against ultraviolet light is described.