Premium
INACTIVATION OF LIPID‐CONTAINING VIRUSES BY HYDROPHOBIC PHOTOSENSITIZERS AND NEAR‐ULTRAVIOLET RADIATION
Author(s) -
Snipes Wallace,
Keller Gregory,
Woog John,
Vickroy Tom,
Deering Reginald,
Keith Alec
Publication year - 1979
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1979.tb07767.x
Subject(s) - singlet oxygen , phenothiazine , sodium azide , chemistry , acridine , photochemistry , oxygen , acridine orange , diphenylamine , membrane , lipid bilayer , liposome , photosensitizer , biochemistry , biophysics , organic chemistry , biology , apoptosis , pharmacology
—The hydrophobic photosensitizers acridine and phenothiazine inactivate the lipid‐contnining viruses PM2,φ6, and herpes simplex when samples are illuminated with near‐UV radiation. φ23–1‐ a . which is insensitive to organic solvents and presumably contains no lipids. is not inactivated under comparable conditions. For acridinc, the inactivation of virus requires that oxygen be present and is inhibited by sodium azide, implicating the involvement of singlet oxygen. For phenothiazine, oxygen is not required for photosensitized inactivation. Treatment of PM2 with acridine and near‐UV light caused a complete disruption of the virion, as determined by sucrose gradient analysis of treated and untreated samples. These data and related observations suggest that lipid‐containing viruses are inactivated through photosensitized membrane damage.