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EFFECT OF PHOTOREACTIVATING LIGHT ON LETHAL AND PRE‐MUTATIONAL UV LESIONS IN ESCHERICHIA COLI WP2 s CARRYING THE R46 MUTATOR PLASMID
Author(s) -
Todd P. A.,
Banfi E.,
Eftimiadi C.,
MontiBragadin C.
Publication year - 1979
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1979.tb07766.x
Subject(s) - photolyase , plasmid , escherichia coli , biology , strain (injury) , dna , microbiology and biotechnology , pyrimidine dimer , dna repair , mutation , genetics , gene , anatomy
—An excision‐deficient E. coli strain carrying the R46 mutator plasmid showed a different response towards photo‐reactivation after UV irradiation than the same strain without plasmid. While the photoreactivation of lethal lesions was comparable in both strains, the number of UV‐induced mutants per 10 6 survivors was slightly reduced for the plasmid bearing strain by photoreactivating light at UV fluences below 60 mJ/m 2 but increased at higher fluences. To explain this it is proposed that some UV photoproduct(s) of DNA other than cyclobutane dipyrimidine dimers are pre‐mutational lesions for error‐prone DNA repair by the plasmid, P‐repair, but not for SOS‐repair.