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7‐ cis ‐PORPHYROPSIN FROM 7‐ cis‐ 3‐DEHYDRORETINAL AND CATTLE OPSIN *
Author(s) -
Matsumoto Hiroyuki,
Asato Alfred E.,
Liu Robert S. H.
Publication year - 1979
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1979.tb07751.x
Subject(s) - visual pigments , opsin , pigment , hydroxylamine , photoisomerization , solvent , isomerization , elution , rhodopsin , chemistry , absorption (acoustics) , hydroxylapatite , column chromatography , photochemistry , chromatography , materials science , organic chemistry , biochemistry , retinal , catalysis , composite material , enzyme
7‐ cis ‐3‐Dehydroretinol, prepared by photoisomerization of the all‐trans isomer in a polar solvent, was found to combine with cattle opsin to form a visual pigment analogue with an absorption maximum at 464 nm. The pigment is only moderately stable in hydroxylamine or in Ammonyx LO. and cannot be purified by column chromatography using Ammonyx LO. 9‐ cis ‐Porphyropsin, prepared in a manner similar to that reported by Azuma et al ., is stable and has been purified by passing through a hydroxylapatite column. Three fractions were collected with eluant of increasing ionic strength. All fractions exhibit similar absorption properties with Λ max at 498 nm. These results further indicate that the binding of opsin is a flexible one.