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PHOTON COUNTING SPECTRAL ANALYZING SYSTEM OF EXTRA‐WEAK CHEMI‐ AND BIOLUMINESCENCE FOR BIOCHEMICAL APPLICATIONS
Author(s) -
Inaba Humio,
Shimizu Yoshiaki,
Tsuji Yasuhiro,
Yamacishi Akio
Publication year - 1979
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1979.tb07131.x
Subject(s) - spectrum analyzer , spectrometer , spectral power distribution , spectral sensitivity , photon , photon counting , noise (video) , optics , grating , bioluminescence , filter (signal processing) , spectral line , biological system , signal (programming language) , chemistry , analytical chemistry (journal) , physics , wavelength , computer science , chromatography , artificial intelligence , image (mathematics) , computer vision , biology , programming language , biochemistry , astronomy
— The systematic study of the method for measuring the spectral distribution of extra‐weak light signals, such as from biochemical systems and living tissues involving chemi‐ and bio‐energized processes, led to the design and construction of a new type of spectrometer, called the filter spectral analyzer system, incorporated with the high‐sensitivity photon counting technique. Experimental tests to examine the operational characteristics of this filter spectral analyzer system and comparisons of the signal‐to‐noise ratio with a conventional grating spectrometer were made by accomplishing the spectral analysis of low‐level light sources. The measurement of the spectra of very weak chemilumines‐cence accompanying various kinds of chemical and biological processes, including autoxidation and enzymatic reaction were performed successfully.