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CHEMILUMINESCENCE FROM EUKARYOTIC AND PROKARYOTIC CELLS: REDUCING POTENTIAL AND OXYGEN REQUIREMENTS *
Author(s) -
Allen Robert C.
Publication year - 1979
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1979.tb07129.x
Subject(s) - chemiluminescence , chemistry , biochemistry , pentose phosphate pathway , oxidative phosphorylation , metabolism , bioluminescence , bacteria , light emission , oxygen , redox , reactive oxygen species , photochemistry , glycolysis , biology , organic chemistry , physics , optoelectronics , genetics
— The postphagocytic microbicidal activity of polymorphonuclear leukocytes (PMNL), the effector phagocytes of the acute inflammatory response, is metabolically characterized by increased glucose oxidation via the hexose monophosphate shunt and by increased non‐mitochondrial oxygen consumption. These metabolic alterations result from the phagocytic activation of a membrane‐associated NADPH:O, oxidoreductase. The products of univalent reduction of O 2 by this enzyme, O ‐ 2 and H + , may further react to yield potential microbicidal oxidants such as H 2 O 2 , OC1 ‐ ‐, OH, and 1 A g O 2 . The microbicidal activity of PMNL is associated with the generation of luminescence. This chemiluminescence correlates with the metabolic generation of reducing potential and O 2 consumption, and is proposed to originate from the relaxation of the electronically excited products of microbicidal oxidation. The importance of O 2 in microbicidal metabolism will be considered, and data demonstrating the O 2 ‐dependence of PMNL‐chemiluminescence are presented. The phenomenon of chemiluminescence from non‐bioluminescent bacteria is also described, and preliminary data from luminescence studies of Streptococcus faecalis are presented. The relationship of light emission to bacterial metabolism and O 2 toxicity is considered. Evidence for the bacterial generation of O ‐ 2 is presented, and the role of O ‐2 and H 2 O 2 in oxidative reactions productive of electronic excited molecular products is discussed.