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DNA STRAND BREAKS IN MAMMALIAN CELLS EXPOSED TO LIGHT IN THE PRESENCE OF RIBOFLAVIN AND TRYPTOPHAN
Author(s) -
Hoffmann M. Edwiges,
Meneghini Rogerio
Publication year - 1979
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1979.tb07052.x
Subject(s) - riboflavin , xeroderma pigmentosum , tryptophan , dna , fluorescence , hydrogen peroxide , chemistry , microbiology and biotechnology , biochemistry , biophysics , dna damage , photochemistry , biology , amino acid , physics , quantum mechanics
— When mammalian cells were exposed to visible‐fluorescent light or near‐UV light in the medium containing riboflavin and L‐tryptophan, single‐strand breaks appeared in their DNA. This did not occur if either riboflavin or tryptophan was omitted from the medium. The same effect was observed when cells were added to the pre‐irradiated medium, indicating that a stable photoproduct was responsible. The induced DNA lesions were shown to be equally repairable in both excision proficient and defective (xeroderma pigmentosum) human cell lines. The active photoproduct formed was shown to be hydrogen peroxide. The possible relationship between these results and the near‐UV induced killing of mammalian cells is discussed.