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DIRECT PHOTO‐AFFINITY LABELING WITH CYCLIC NUCLEOTIDES: ACTION SPECTRA
Author(s) -
Antonoff Ross S.,
Ferguson J. J.
Publication year - 1978
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1978.tb07637.x
Subject(s) - guanosine , nucleotide , chemistry , covalent bond , cyclic nucleotide , ligand (biochemistry) , action spectrum , cyclic guanosine monophosphate , receptor , guanosine monophosphate , adenosine , stereochemistry , biophysics , biochemistry , photochemistry , biology , organic chemistry , nitric oxide , gene
— When [ 3 H]‐cyclic adenosine 3′,5′‐monophosphate and [ 3 H]‐cyclic guanosine 3′,5′‐monophos‐phate are irradiated at varying UV wavelengths in the presence of cyclic nucleotide receptor proteins, photo‐incorporation is maximal in the region of 280 nm. This suggests that covalent coupling occurs by means of photo‐activation of an entity other than the ligand cyclic nucleotide. The action spectra more closely resemble the absorbtion spectrum of the reacting protein, making it likely that absorhtion of radiant energy by components of the protein receptor leads to photo‐affinity labeling.