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SIMILAR SPECTRA FOR THE INACTIVATION BY MONOCHROMATIC LIGHT OF TWO DISTINCT LEUCINE TRANSPORT SYSTEMS IN ESCHERICHIA COLI
Author(s) -
Robb F. T.,
Hauman J. H.,
Peak M. J.
Publication year - 1978
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1978.tb07630.x
Subject(s) - monochromatic color , leucine , escherichia coli , chemistry , wavelength , kinetics , spectral line , biophysics , biochemistry , biology , physics , amino acid , optics , quantum mechanics , gene , astronomy
—Kinetics of inactivation of two separate leucine transport systems (leucine specific and LIV) in E. coli by seven wavelengths of monochromatic light have been studied. Loss of leucine uptake is not due to generalized membrane damage causing non‐specific leucine leakage. Inactivations are usually exponential but some wavelengths show shoulders at low doses. Two‐component spectra between 254 and 435 nm occur for both transport systems. Inactivation is most efficient at 290 nm and a second peak occurs at 365 nm. Both leucine transport systems are inactivated similarly.