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DIFFERENTIAL EFFECTS OF TRYPTOPHAN PHOTOPRODUCTS AND H 2 O 2 ON THE GROWTH OF MOUSE EMBRYONIC FIBROBLASTS *
Author(s) -
Zigman S.,
Hare J. D.,
Yulo T.,
Ennist D.
Publication year - 1978
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1978.tb07601.x
Subject(s) - hydrogen peroxide , embryonic stem cell , tryptophan , thymidine , dna , chemistry , yield (engineering) , salt (chemistry) , oxygen , biochemistry , microbiology and biotechnology , biology , amino acid , organic chemistry , gene , materials science , metallurgy
. Exposure of dilute solutions of L‐tryptophan in balanced salt mixtures to near‐ultraviolet light (ca. 320–390nm) yield several photoproducts capable of inhibiting the growth of cultured mouse embryonic fibroblasts. Recent literature suggested a need to test the possibility that the major toxic photoproduct is hydrogen peroxide, and therefore several lines of experimentation were pursued. On the basis of chemical and morphological studies and an analysis of the uptake of 3 H‐thymidine by these cells and its incorporation into their DNA, it is concluded that sufficient hydrogen peroxide could not be generated without the addition of high concentrations of oxygen to influence the growth or DNA synthesis of mouse embryonic fibroblasts.