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BLUE LIGHT EFFECT ON CHLOROPHYLL FORMATION IN CHLORELLA PROTOTHECOIDES
Author(s) -
OhHama Tamko,
Hase EIJI
Publication year - 1978
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1978.tb07588.x
Subject(s) - greening , action spectrum , darkness , cycloheximide , chlorophyll , blue light , irradiation , chlorella , chemistry , white light , saturation (graph theory) , botany , biophysics , chlorophyll a , biochemistry , photochemistry , biology , algae , optics , protein biosynthesis , ecology , physics , mathematics , combinatorics , nuclear physics
The effectiveness of monochromatic light on chlorophyll formation over a range of 420–80 nm was examined in the regreening cells of Chlorella protothecoides in the presence of chloro‐phenyl dimethylurea (CMU). An action spectrum showed two maxima with a minimum near 450 nm. At the most effective wavelength examined (444 nm), 0.2 W. m ‐2 was sufficient for half saturation. The activity of 5‐aminolevulinic acid (ALA) production was examined in the greening cells under irradiation with white, blue and red light. The activity was always limited by availability of substrate, especially in the case where the greening cells were incubated with cycloheximide or transferred to darkness. Decay of the activity in these cases was delayed by provision of organic compounds such as glycine, pyruvate or glucose. The effectiveness of blue light on ALA* production observed in the presence of CMU was inferred to be brought about by the increased availability of endogenous substrates.