Premium
CHARACTERIZATION OF THE 32 ,000 DALTON MEMBRANE PROTEIN—I. EARLY SYNTHESIS DURING PHOTOINDUCED PLASTID DEVELOPMENT OF SPIRODELA
Author(s) -
Reisfeld AVI,
Gressel Jonathan,
Jakob Karl M.,
Edelman Marvin
Publication year - 1978
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1978.tb07581.x
Subject(s) - plastid , greening , protein biosynthesis , methionine , kinetics , messenger rna , chemistry , biochemistry , membrane , biophysics , rna , biosynthesis , biology , chloroplast , amino acid , gene , ecology , physics , quantum mechanics
The kinetics of 35 S methionine incorporation into soluble and membrane proteins during the transition from steady state dark growth to greening was studied in Spirodela . A sharp increase in the rate of incorporation occurred at 3 h, which was several h before major increases in chlorophyll were apparent. Part of this enhanced incorporation was due to enhanced synthesis of a 32 ,000 dalton membrane protein. This synthesis was paralleled by a temporal increase in in uitro template capacity for this protein and an increase in 0.5 × 10 6 dalton plastid messenger RNA.