Premium
ANTIGENICITY OF DNA INDUCED BY PHOTOADDITION OF 8‐METHOXYPSORALEN
Author(s) -
Zarebska Z.,
JarȩbekArzelska M.,
Rzȩsa G.,
Chorzelski T.
Publication year - 1978
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1978.tb07562.x
Subject(s) - antiserum , dna , microbiology and biotechnology , antigenicity , immunodiffusion , chemistry , immunofluorescence , ouchterlony double immunodiffusion , biology , antibody , biochemistry , immunology
— Rabbits immunized with sonicated DNA UV‐irradiated (365 nm) in the presence of 8‐methoxypsoralen (8‐MOP) produced a specific antiserum directed against DNA‐8‐MOP‐photoadduct. None of the long chain DNA preparations modified photochemically in the same way elicited an immunological response, although all of them gave a positive immunodiffusion test on agarose with the antiserum directed against DNA‐8‐MOP‐photoadduct. A positive reaction has also been demonstrated in the indirect immunofluorescence test, using as a source of cellular native DNA Crithidia luciliae cells treated with 8‐MOP and irradiated at 365 nm (UVA). A possible use of the specific antiserum for detecting the formation of DNA‐8‐MOP‐photoadduct, in the skin and/or lymphocytes of patients with psoriasis treated by combination of 8‐MOP and UVA irradiation, is suggested.